Biomarker Discovery:Article Title: A class I PI3K signalling network regulates primary cilia disassembly in normal physiology and disease
Article Snippet: .. Investigators were not blinded for cell biology experiments. pAKT(S473), Cell Signalling Technologies (#9271) pAKT(T308), Cell Signalling Technologies (#9275) AKT, Cell Signalling Technologies (#9272) PI3Ka, Cell Signalling Technologies (#4249) 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. pPRAS40(S246), Cell Signalling Technologies (#2640) Ki67, Cell Signalling Technologies (Alexa Fluor 488 Conjugate, #11882) pS6RP(Ser240/244), Cell Signalling Technologies (#5364) ARL13B, Abcam (ab136648) AlexaFluor647-conjugated anti-ERG, Abcam (ab196149) GAPDH, Abcam (ab8245) Pericentrin, Abcam (ab4448) ARL13B, Proteintech (17711-1-AP) CEP170, Sigma-Aldrich (HPA042151) "-tubulin, Sigma-Aldrich (T5326) Acetylated !-tubulin, Sigma-Aldrich (T7451) HA, BioLegend (HA.11) PI(3,4)P2, Echelon Biosciences (Z-P034) PI(3,4,5)P3, Echelon Biosciences (Z-P345b) SMO, Santa Cruz Biotechnology (sc-166685) Alexa-Fluor-488/568/594/647-conjugated mouse and rabbit secondary antibodies, ThermoFisher Scientific AlexaFluor568-conjugated isolectin GS-B4, ThermoFisher Scientific (I21412) HRP-conjugated mouse (NXA931V) and rabbit (NA934V) secondary antibodies, Cytiva All antibodies have been validated either by the company or ourselves. hTERT-RPE1 cells were purchased from ATCC (Manassas, VA, USA; CRL-4000) HEK293 (LentiX) cells were purchased from Clontech (Mountain View, CA, USA, NC9834960) MCF10A cells were purchased from ATCC (CRL-10317) A549 cells were purchased from ATCC (CCL-185) BPH1 cells were purchased from DSMZ (ACC 143, ref.14602) Immortalised Pik3ca+/+ and Pik3ca-/- MEFs were generated and described previously (Foukas et al, PNAS 2010) The authenticity of hTERT-RPE1 cells is tested by ATCC using short tandem repeat analysis, immunocytochemistry for pancytokeratin and flow cytometry for Ep-16 expression. ..
Generated:Article Title: A class I PI3K signalling network regulates primary cilia disassembly in normal physiology and disease
Article Snippet: .. Investigators were not blinded for cell biology experiments. pAKT(S473), Cell Signalling Technologies (#9271) pAKT(T308), Cell Signalling Technologies (#9275) AKT, Cell Signalling Technologies (#9272) PI3Ka, Cell Signalling Technologies (#4249) 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. pPRAS40(S246), Cell Signalling Technologies (#2640) Ki67, Cell Signalling Technologies (Alexa Fluor 488 Conjugate, #11882) pS6RP(Ser240/244), Cell Signalling Technologies (#5364) ARL13B, Abcam (ab136648) AlexaFluor647-conjugated anti-ERG, Abcam (ab196149) GAPDH, Abcam (ab8245) Pericentrin, Abcam (ab4448) ARL13B, Proteintech (17711-1-AP) CEP170, Sigma-Aldrich (HPA042151) "-tubulin, Sigma-Aldrich (T5326) Acetylated !-tubulin, Sigma-Aldrich (T7451) HA, BioLegend (HA.11) PI(3,4)P2, Echelon Biosciences (Z-P034) PI(3,4,5)P3, Echelon Biosciences (Z-P345b) SMO, Santa Cruz Biotechnology (sc-166685) Alexa-Fluor-488/568/594/647-conjugated mouse and rabbit secondary antibodies, ThermoFisher Scientific AlexaFluor568-conjugated isolectin GS-B4, ThermoFisher Scientific (I21412) HRP-conjugated mouse (NXA931V) and rabbit (NA934V) secondary antibodies, Cytiva All antibodies have been validated either by the company or ourselves. hTERT-RPE1 cells were purchased from ATCC (Manassas, VA, USA; CRL-4000) HEK293 (LentiX) cells were purchased from Clontech (Mountain View, CA, USA, NC9834960) MCF10A cells were purchased from ATCC (CRL-10317) A549 cells were purchased from ATCC (CCL-185) BPH1 cells were purchased from DSMZ (ACC 143, ref.14602) Immortalised Pik3ca+/+ and Pik3ca-/- MEFs were generated and described previously (Foukas et al, PNAS 2010) The authenticity of hTERT-RPE1 cells is tested by ATCC using short tandem repeat analysis, immunocytochemistry for pancytokeratin and flow cytometry for Ep-16 expression. ..
Immunocytochemistry:Article Title: A class I PI3K signalling network regulates primary cilia disassembly in normal physiology and disease
Article Snippet: .. Investigators were not blinded for cell biology experiments. pAKT(S473), Cell Signalling Technologies (#9271) pAKT(T308), Cell Signalling Technologies (#9275) AKT, Cell Signalling Technologies (#9272) PI3Ka, Cell Signalling Technologies (#4249) 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. pPRAS40(S246), Cell Signalling Technologies (#2640) Ki67, Cell Signalling Technologies (Alexa Fluor 488 Conjugate, #11882) pS6RP(Ser240/244), Cell Signalling Technologies (#5364) ARL13B, Abcam (ab136648) AlexaFluor647-conjugated anti-ERG, Abcam (ab196149) GAPDH, Abcam (ab8245) Pericentrin, Abcam (ab4448) ARL13B, Proteintech (17711-1-AP) CEP170, Sigma-Aldrich (HPA042151) "-tubulin, Sigma-Aldrich (T5326) Acetylated !-tubulin, Sigma-Aldrich (T7451) HA, BioLegend (HA.11) PI(3,4)P2, Echelon Biosciences (Z-P034) PI(3,4,5)P3, Echelon Biosciences (Z-P345b) SMO, Santa Cruz Biotechnology (sc-166685) Alexa-Fluor-488/568/594/647-conjugated mouse and rabbit secondary antibodies, ThermoFisher Scientific AlexaFluor568-conjugated isolectin GS-B4, ThermoFisher Scientific (I21412) HRP-conjugated mouse (NXA931V) and rabbit (NA934V) secondary antibodies, Cytiva All antibodies have been validated either by the company or ourselves. hTERT-RPE1 cells were purchased from ATCC (Manassas, VA, USA; CRL-4000) HEK293 (LentiX) cells were purchased from Clontech (Mountain View, CA, USA, NC9834960) MCF10A cells were purchased from ATCC (CRL-10317) A549 cells were purchased from ATCC (CCL-185) BPH1 cells were purchased from DSMZ (ACC 143, ref.14602) Immortalised Pik3ca+/+ and Pik3ca-/- MEFs were generated and described previously (Foukas et al, PNAS 2010) The authenticity of hTERT-RPE1 cells is tested by ATCC using short tandem repeat analysis, immunocytochemistry for pancytokeratin and flow cytometry for Ep-16 expression. ..
Flow Cytometry:Article Title: A class I PI3K signalling network regulates primary cilia disassembly in normal physiology and disease
Article Snippet: .. Investigators were not blinded for cell biology experiments. pAKT(S473), Cell Signalling Technologies (#9271) pAKT(T308), Cell Signalling Technologies (#9275) AKT, Cell Signalling Technologies (#9272) PI3Ka, Cell Signalling Technologies (#4249) 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. pPRAS40(S246), Cell Signalling Technologies (#2640) Ki67, Cell Signalling Technologies (Alexa Fluor 488 Conjugate, #11882) pS6RP(Ser240/244), Cell Signalling Technologies (#5364) ARL13B, Abcam (ab136648) AlexaFluor647-conjugated anti-ERG, Abcam (ab196149) GAPDH, Abcam (ab8245) Pericentrin, Abcam (ab4448) ARL13B, Proteintech (17711-1-AP) CEP170, Sigma-Aldrich (HPA042151) "-tubulin, Sigma-Aldrich (T5326) Acetylated !-tubulin, Sigma-Aldrich (T7451) HA, BioLegend (HA.11) PI(3,4)P2, Echelon Biosciences (Z-P034) PI(3,4,5)P3, Echelon Biosciences (Z-P345b) SMO, Santa Cruz Biotechnology (sc-166685) Alexa-Fluor-488/568/594/647-conjugated mouse and rabbit secondary antibodies, ThermoFisher Scientific AlexaFluor568-conjugated isolectin GS-B4, ThermoFisher Scientific (I21412) HRP-conjugated mouse (NXA931V) and rabbit (NA934V) secondary antibodies, Cytiva All antibodies have been validated either by the company or ourselves. hTERT-RPE1 cells were purchased from ATCC (Manassas, VA, USA; CRL-4000) HEK293 (LentiX) cells were purchased from Clontech (Mountain View, CA, USA, NC9834960) MCF10A cells were purchased from ATCC (CRL-10317) A549 cells were purchased from ATCC (CCL-185) BPH1 cells were purchased from DSMZ (ACC 143, ref.14602) Immortalised Pik3ca+/+ and Pik3ca-/- MEFs were generated and described previously (Foukas et al, PNAS 2010) The authenticity of hTERT-RPE1 cells is tested by ATCC using short tandem repeat analysis, immunocytochemistry for pancytokeratin and flow cytometry for Ep-16 expression. ..
Expressing:Article Title: A class I PI3K signalling network regulates primary cilia disassembly in normal physiology and disease
Article Snippet: .. Investigators were not blinded for cell biology experiments. pAKT(S473), Cell Signalling Technologies (#9271) pAKT(T308), Cell Signalling Technologies (#9275) AKT, Cell Signalling Technologies (#9272) PI3Ka, Cell Signalling Technologies (#4249) 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. pPRAS40(S246), Cell Signalling Technologies (#2640) Ki67, Cell Signalling Technologies (Alexa Fluor 488 Conjugate, #11882) pS6RP(Ser240/244), Cell Signalling Technologies (#5364) ARL13B, Abcam (ab136648) AlexaFluor647-conjugated anti-ERG, Abcam (ab196149) GAPDH, Abcam (ab8245) Pericentrin, Abcam (ab4448) ARL13B, Proteintech (17711-1-AP) CEP170, Sigma-Aldrich (HPA042151) "-tubulin, Sigma-Aldrich (T5326) Acetylated !-tubulin, Sigma-Aldrich (T7451) HA, BioLegend (HA.11) PI(3,4)P2, Echelon Biosciences (Z-P034) PI(3,4,5)P3, Echelon Biosciences (Z-P345b) SMO, Santa Cruz Biotechnology (sc-166685) Alexa-Fluor-488/568/594/647-conjugated mouse and rabbit secondary antibodies, ThermoFisher Scientific AlexaFluor568-conjugated isolectin GS-B4, ThermoFisher Scientific (I21412) HRP-conjugated mouse (NXA931V) and rabbit (NA934V) secondary antibodies, Cytiva All antibodies have been validated either by the company or ourselves. hTERT-RPE1 cells were purchased from ATCC (Manassas, VA, USA; CRL-4000) HEK293 (LentiX) cells were purchased from Clontech (Mountain View, CA, USA, NC9834960) MCF10A cells were purchased from ATCC (CRL-10317) A549 cells were purchased from ATCC (CCL-185) BPH1 cells were purchased from DSMZ (ACC 143, ref.14602) Immortalised Pik3ca+/+ and Pik3ca-/- MEFs were generated and described previously (Foukas et al, PNAS 2010) The authenticity of hTERT-RPE1 cells is tested by ATCC using short tandem repeat analysis, immunocytochemistry for pancytokeratin and flow cytometry for Ep-16 expression. ..
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